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  • ACTIN(鼠抗)

    货号:GB12001

    品牌:Servicebio

    价格:¥160

    规格: 100μl

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    Product Information

     

     

    Description

    beta Actin mouse monoclonal

    Protein full name
    Actin, cytoplasmic 1

    Synonyms

    ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin
    Immunogen

    KLH conjugated Synthetic peptide corresponding to Mouse β-Actin

    Isotype
    IgG
    Purity
    Affinity purification
    Predicted MW.

    42 kDa

    Observed MW.
    45 kDa
    Subcellular location
    Cytoplasm
    Uniprot ID
    P60709,P60710,P60711
     

     


     

    Applications

     

     

    Applications
    WB

    IHC,IF

     
    Species

    Human,Rat,Mouse

    Human,Rat,Mouse

     
    Dilution

    1:1000-1:2000

    1:400-1:2000
     
    Positive tissue

    Hela,Raw264.7,liver,brain

    Liver,lung,stomach

     

     


     

    Background

     

     

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

       


     

    Images

     

     

    image.png

    Western blot analysis of β-Actin (GB12001) at dilution of 1:1000.

    Lane 1:Hela cell lysate

    Lane 2:Raw264.7 cell lysate

    Lane 3:C6 cell lysate

    Lane 4:HCT116 tumor tissue lysate

    Lane 5:HepG2 tumor tissue lysate

    Lane 6:Mouse liver tissue lysate

    Lane 7:Mouse brain tissue lysate

    Lane 8:Rat liver tissue lysate

    Lane 9:Rat brain tissue lysate

     
     
    image.png

    Immunohistochemistry analysis of paraffin-embedded human liver using β-Actin(GB12001) at dilution of 1:1300

     
     
    image.png

    Immunohistochemistry analysis of paraffin-embedded mouse ovary using β-Actin(GB12001) at dilution of 1:400

     


     

    Storage

     

     

    Storage
    Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
    Storage Buffer
    PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

     


     

    NOTE: This product is intended for research only.

     

     

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